Changes in proximity labelled proteins due to binding of ligands and inhibitors to the EGFR. (IMAGE)
Caption
A) Heatmap diagram showing biotinylation changes in DMSO (without ligand and drug addition), EGF (ligand) and EGF+Gefitinib (ligand + drug) treated zones. Cells were epithelial cancer cells (A431 cells). Black letters in proteins are known EGFR-interacting proteins, blue letters are EGFR-interacting proteins newly identified in the present study.
B) Diagram depicting exPPI changes in EGFR. In epithelial cancer cells, EGFR and EEF1A1 are in close proximity when EGF is not bound; EGF binding increases the degree of proximity between INSR and EGFR and drug binding there increases the degree of proximity between PTK7 and EGFR.
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By Kohdai Yamada, Sawasaki Tatsuya（Ehime University）
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