Imaging neural activity in singing flies (VIDEO)
Caption
Using a combination recording device and microscope, researchers can image the neural activity in the fly’s nerve cord while the insect sings. This video shows an example trial of calcium imaging of dPR1 neurons. Top left: a bottom view of the fly. The left side of the image corresponds to the right side of the fly. Bottom left: the microphone signal for the right wing. Top right: raw fluorescent signals averaged across z-planes. The left side of the image corresponds to the left side of the ventral nerve cord. Bottom right: schematic of the imaged volume. Red boxes represent the timing of optogenetic stimulation. Optogenetic stimulation acutely increased calcium signals of dPR1 neurons as well as the amount of song, consistent with a role of dPR1 in song production.
Credit
Shiozaki et al. DOI: 10.1101/2022.12.14.520499
Usage Restrictions
Please use credit provided.
License
Original content