Model showing the use of modified OLE1 in PUSH-PULL-PROTECT strategy for increasing oil accumulation in plant cells (IMAGE)
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Model showing the use of modified OLE1 in PUSH-PULL-PROTECT strategy for increasing oil accumulation in plant cells. The strategy combines modifications to push carbon into the production of triacylglycerol (TAG), a form of oil that is stored in lipid droplets (LD), using an efficient mouse diacylglycerol O-acyltransferase 2 gene (mD). In this study, the scientists explored ways to modify oleosin, a protein that protects lipid droplets from degradation. A. With oleosin variants SiO or SiCO, there is still significant oil degradation. B. Variant OLE1 is more resistant to breakdown, so it provides more protection and results in decreased oil degradation.
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Sanket Anaokar/Brookhaven National Laboratory
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