Building images photon-by-photon to increase the information content provided by microscopes. (IMAGE)

SPIE--International Society for Optics and Photonics

Building images photon-by-photon to increase the information content provided by microscopes.

Caption

(a) Comparison of confocal and ISM fluorescence lifetime maps of tubulin filaments of a HeLa cell. (b) Comparison of raw STED and ISM-SPLIT-STED image of tubulin filaments. (c) Phasor representation of two dyes with the same lifetime and overlapping excitation spectra, individually excited by two different laser pulses (top). Phasor representation of the mixed contributions of the two dyes (bottom). (d) Channel separation using time gating (left) and phasor separation (right). Due to crosstalk, only phasor separation successfully distinguishes the two dyes. 

Credit

Tortarolo, Zunino, et al., doi 10.1117/1.AP.6.1.016003.

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