Fig. 2 (IMAGE)

Kanazawa University

Fig. 2

Caption

High-speed AFM observation and simulation of the structural dynamics of the HECT domain of E6AP.
(A) HS-AFM image of the HECT domain. Spherical (white dotted line) and oval (blue dotted line) conformational states were observed. (B) Simulated transition process of the structural state of the HECT domain and its pseudo AFM image. (C) Ubiquitin (Ub) is transferred from E2 to E3 (HECT domain). After the addition of ubiquitin-containing E2 (E2-Ub), E2-Ub (white arrow) binds to the HECT domain, and after E2 dissociates, a small particle (blue arrow) that appears to be ubiquitin is added to the HECT domain. 

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© 2023 Takeda, et al., Published by American Chemical Society

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