Working principle of the CRONT. (IMAGE)

Light Publishing Center, Changchun Institute of Optics, Fine Mechanics And Physics, CAS

Working principle of the CRONT.

Caption

a, The diagrammatic sketch of the three components in the solution: DNA@AuNS conjugate, CRISPR/Cas12a complex, and target ssDNA. b, Optical setup, the BS, SPF, and TL are beam splitter, short pass filter, and tube lens (f=200 mm), respectively. Additional details of the setup are provided in the Materials and Methods section. c, Dispersion of the three components in the solution without optical heating. d, Optothermal net force (FNet) induced migration and DNA@AuNS conjugate cleavage upon optical heating, the heating laser power is 0.5 mW. e, Observation of the cleavage after the optical heating is switched off.

Credit

by Jiajie Chen, Zhi Chen, Changle Meng, Jianxing Zhou, Yuhang Peng, Xiaoqi Dai, Jingfeng Li, Yili Zhong, Xiaolin Chen, Wu Yuan, Ho-Pui Ho, Bruce Zhi Gao, Junle Qu, Xueji Zhang, Han Zhang & Yonghong Shao

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License

CC BY

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