CRONT system for nucleotide detection and identification. (IMAGE)
Caption
a, A single DNA@AuNS is captured by the CRONT at the laser heating region. The heating laser is turned off at 28.8s, and cleavage is observed afterward. b, Trapping stiffness measurements at varying laser powers in x/y direction, with the dashed line denoting the maximum stiffness at 0.5 mW. c, Position distribution of the trapped single DNA@AuNS at 0.5 mW. d, Light intensity variation of a trapped DNA@AuNS during the laser activation. The target ssDNA is from part of the Monkeypox (MP) virus sequence. Frames were recorded using dark-field microscopy, and the scale bar is 2 μm. e, Cleavage probability of the DNA@AuNS at different target ssDNA (MP) concentrations. f, Cleavage probability at different crRNA and target ssDNA combination groups (A-E) for specificity test, the target ssDNA concentrations is 250 fM. g, Cleavage probability of the DNA@AuNS at different target dsDNA (MP) concentrations. The optical power set as 0.5 mW in a, c-g. h, Cleavage probability of the DNA@AuNS under dsDNA at a lower optical power of 0.16 mW, the inset indicates the temperature distribution. Each capturing event was conducted for 2 minutes, and each data point comprised 10-17 capturing events over a 40-minute period. Each concentration was tested three times. The PEG mass fraction is 10%. The concentration of AuNS and Cas12a is 0.5 μM and 0.125 nM respectively.
Credit
by Jiajie Chen, Zhi Chen, Changle Meng, Jianxing Zhou, Yuhang Peng, Xiaoqi Dai, Jingfeng Li, Yili Zhong, Xiaolin Chen, Wu Yuan, Ho-Pui Ho, Bruce Zhi Gao, Junle Qu, Xueji Zhang, Han Zhang & Yonghong Shao
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Credit must be given to the creator.
License
CC BY