Figure 3 (IMAGE) Institute for Basic Science Caption A) Traffic jams at the intersection of the cytoskeleton in a local area within a cell. This figure captures a traffic congestion event evidenced by cargo crowding and suppressed mobility. (left) Static background removed (SBR-) iSCAT and (right) time-differential (TD-) iSCAT imaging techniques. Notably, both bright and dark spots in the SBR-iSCAT image (highlighted by numbered yellow circles) correspond to dynamic cargos observed in the TD-iSCAT image. Within the red circle in the TD-iSCAT image, a cluster of cargos experiences a bottleneck. B) A total of 83 trajectories of cargo continuously tracked over 500 frames in 9,000 consecutive SBR-iSCAT images are drawn. Among these trajectories, those of 49 cargos displaying sub-diffusive motion are depicted in gray. Credit Institute for Basic Science Usage Restrictions Attribution Required License Original content Disclaimer: AAAS and EurekAlert! are not responsible for the accuracy of news releases posted to EurekAlert! by contributing institutions or for the use of any information through the EurekAlert system.