Two-photon imaging (IMAGE) University of Cincinnati Caption 3D reconstruction of microscope images of brain organoids stained with the new three-arm probe are exposed to one-photon (top) or two-photon (bottom) excitation. The two-photon process provides more detail through its fluorescent intensity. Credit Photo provided by Jiajie Diao and Yuije Sun. Usage Restrictions None License Original content Disclaimer: AAAS and EurekAlert! are not responsible for the accuracy of news releases posted to EurekAlert! by contributing institutions or for the use of any information through the EurekAlert system.