An international research team led by Monash University has uncovered a new technique that could speed up recovery from bone replacements by altering the shape and nucleus of individual stem cells.
The research collaboration involving Monash University, the Melbourne Centre for Nanofabrication, CSIRO, the Max Planck Institute for Medical Research and the Swiss Federal Institute of Technology in Lausanne, developed micropillar arrays using UV nanoimprint lithography that essentially 'trick' the cells to become bone.
Nanoimprint lithography allows for the creation of microscale patterns with low cost, high throughput and high resolution.
When implanted into the body as part of a bone replacement procedure, such as a hip or knee, researchers found these pillars - which are 10 times smaller than the width of a human hair - changed the shape, nucleus and genetic material inside stem cells.
Not only was the research team able to define the topography of the pillar sizes and the effects it had on stem cells, but they discovered four times as much bone could be produced compared to current methods.
The findings were published in Advanced Science.
"What this means is, with further testing, we can speed up the process of locking bone replacements with surrounding tissue, in addition to reducing the risks of infection," Associate Professor Jessica Frith from Monash University's Department of Materials Science and Engineering said.
"We've also been able to determine what form these pillar structures take and what size they need to be in order to facilitate the changes to each stem cell, and select one that works best for the application."
Researchers are now advancing this study into animal model testing to see how they perform on medical implants.
Engineers, scientists and medical professionals have known for some time that cells can take complex mechanical cues from the microenvironment, which in turn influences their development.
However, Dr Victor Cadarso from Monash University's Department of Mechanical and Aerospace Engineering says their results point to a previously undefined mechanism where 'mechanotransductory signalling' can be harnessed using microtopographies for future clinical settings.
"Harnessing surface microtopography instead of biological factor supplementation to direct cell fate has far-reaching ramifications for smart cell cultureware in stem cell technologies and cell therapy, as well as for the design of smart implant materials with enhanced osteo-inductive capacity," Dr Cadarso said.
Professor Nicolas Voelcker from the Monash Institute of Pharmaceutical Sciences and Director of the Melbourne Centre for Nanofabrication said the study results confirm micropillars not only impacted the overall nuclear shape, but also changed the contents of the nucleus.
"The ability to control the degree of deformation of the nucleus by specifying the architecture of the underlying substrate may open new opportunities to regulate gene expression and subsequent cell fate," Professor Voelcker said.
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Dr Victor Cadarso and Associate Professor Jessica Frith from Monash University's Faculty of Engineering, and Professor Nicolas Voelcker from the Monash Institute of Pharmaceutical Sciences and Adjunct Professor in Materials Science and Engineering, led the study.
They were supported by James Carthew and Margeaux Hodgson-Garms (Materials Science and Engineering, Monash University); Sara Ghavamian and Hazem Abdelmaksoud (Mechanical and Aerospace Engineering, Monash University); Dr Roey Elnathan (Monash Institute of Pharmaceutical Sciences); Stella Aslanoglou (Melbourne Centre for Nanofabrication); Professor Joachim Spatz (Max Planck Institute for Medical Research); Juergen Brugger (Swiss Federal Institute of Technology in Lausanne); and Dr Helmut Thissen (CSIRO).
To download a copy please visit https://onlinelibrary.wiley.com/doi/10.1002/advs.202003186
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Leigh Dawson
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Journal
Advanced Science