image: In this series of snapshots (kymograph), oscillations and waves of gene activity in healthy cells are seen. In cells with mutations in genes such as HES7, DLL3, and LFNG, these patterns of gene activity are stopped or disrupted, causing errors in development. view more
Credit: Ebisuya group/EMBL
Segmentation clock
Our vertebral column is a highly repetitive structure - 33 vertebrae from top to bottom. This arrangement is created in the embryo by the sequential formation of a long row of structures called somites (see image), which later give rise to the vertebrae and ribs. This periodic pattern of somites is created by a group of genes known as the segmentation clock. Molecular interactions within the cell cause the expression of these genes to oscillate, with gene activity rising and falling in a regular pattern over time (see movie below). For each oscillation, another somite is formed. Errors in this segmentation clock can cause hereditary disorders of the vertebrae, such as the rare condition spondylocostal dysostosis (SCD).
The dynamics of the human segmentation clock and related diseases cannot be studied directly in human embryos, so EMBL Research Scientist Mitsuhiro Matsuda and collaborators tried to create a system for studying this process in the lab. They created cell lines that each lacked a gene thought to be the causative mutation of SCD - which can be caused by any of several genes - in different patients. They cultured these cells to create simplified versions of an embryo that show many of the same characteristics. While cells lacking a gene called HES7 failed to show oscillations, cells lacking the genes DLL3 and LFNG surprisingly showed intact oscillations. However, despite oscillations occurring in these cell lines at the single-cell level, they did not properly coordinate across the tissue to form synchronised collective oscillations or travelling waves of gene activity.
Further tests
These experiments demonstrated that the culture system the scientists has created could reveal SCD mutations that had been engineered into otherwise healthy cells. But what about testing patients' cells directly? They established a new cell line from a patient with a mutation in DLL3, and tested it in vitro. As expected, this cell line failed to show travelling waves. To provide the strongest evidence that the DLL3 mutation was the cause, the researchers used the gene editing tool CRISPR-Cas9 to correct the patient's mutation. This restored the normal synchronisation of the segmentation clock in the in vitro tissue, proving that this specific mutation was responsible.
"The segmentation clock, the mechanism underlying the periodic structures of the vertebral column, has been recapitulated in vitro. We also succeeded in evaluating two important properties of the segmentation clock separately: oscillation and synchronisation," says EMBL group leader Miki Ebisuya. "HES7, DLL3, and LFNG were already known as causative genes of SCD. But, for many SCD patients, the causative genes are still unknown. Our next goal is to identify a novel causative gene of SCD by using our newly established in vitro model."
###
Journal
Nature