image: R545 is critical in DNA cleavage of TtAgo, while R486 plays a negligible role. (Left) Catalytic tetrad with plugged-in glutamate finger (yellow). (Right) view more
Credit: Hong Kong University of Science and Technology
Enzymes have clearly defined active sites to allow the substrate molecule to fit intricately. This is often coupled with an enzymatic conformational change prior to the occurrence of the catalysis reaction. For Ago, the catalysis step requires insertion of a "glutamate finger" to form the catalytic plugged-in conformation, which can be stabilized through hydrogen-bonding networks provided by two symmetric positively-charged residues.
For Ago in eukaryotes, these two symmetric positively-charged residues play the identical role that is critical for cleavage. Hence, it was long speculated that the two analogous resides in prokaryotic Ago perform the same critical role in cleavage function. Surprisingly, this study (Fig. 1) showed that in pAgo, only one (Arginine 545) of the two residues is involved in cleavage function. When the other one (Arginine 486) was substituted with other amino acids, the enzyme was still able to maintain its cleavage activity. Based on these results, the study further suggested that R486 may play other roles such as assisting the insertion of the glutamate finger. The discovery of such striking differences in the roles of these symmetric resides between eAgos and pAgos provides novel insights on how the cleavage functions evolve during the evolution journey from prokaryote to eukaryote.
To achieve these results, computational methods combining Quantum Mechanics, Molecular Mechanics, and Molecular Dynamics (QM/MM) were applied to elucidate the cleavage reaction mechanism and identify functional roles of the amino acid residues. This research was made possible by large-scale high-performance computing resources, which were computed equivalent to 10,000 CPU cores for 25 weeks on the Shaheen II Supercomputer at KAUST in collaboration with Prof. Xin GAO's group.
"This research was made possible due to current day computing capabilities and the precision that QM/MM modelling allows for," said Prof. HUANG Xuhui. "Comparing which amino acid residues play a key part in the target DNA/RNA cleavage step in pAgo and eAgo sheds light on how Ago protein evolves from prokaryotes to eukaryotes to cleave DNA/RNA. This information may be useful in ultimately modifying the Ago protein for use as an enhanced gene editing tool in the future," Prof. Huang explained.
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This collaborative study on the bacterial Thermus thermophilus Ago (TtAgo; pAgo) enzyme was led by Prof. HUANG Xuhui, Padma Harilela Associate Professor of Science, from the Department of Chemistry at the Hong Kong University of Science and Technology (HKUST), alongside Prof. ZHANG Yinghai from New York University, and Prof. WANG Yanli from the Chinese Academy of Sciences. The goal behind this research was to identify and contrast the function(s) of key amino acid residues that are present in both pAgo and eAgo.
Details of the methodology and their findings were published in the Proceedings of the National Academy of Sciences of the United States of America (PNAS) journal on December 27, 2018. (DOI: 10.1073/pnas.1817041116)
Journal
Proceedings of the National Academy of Sciences