May 24, 2018 - Earlier this year, the last remaining male Northern White Rhinoceros (NWR) died in captivity, nearly cementing the fate of this subspecies for extinction. In the wild, continuing threats of poaching, habitat destruction, and small population size have contributed to the rhinos' status as critically endangered. Yet, novel conservation efforts that make use of cryopreserved genetic material could save the NWR, and other threatened species, from extinction.
In a study published today in Genome Research, researchers investigated the genetic history of nine NWR cryopreserved cell lines compared to that of a closely related subspecies, the Southern White Rhino (SWR). Genome analyses demonstrated that the NWR and SWR represent two distinct populations that diverge nearly 80,000 years ago, each with fairly high genetic variation compared to other threatened species. Importantly, genetic analyses of variation and inbreeding facilitated identification of cell lines, which may serve as valuable pools of genetic material for genetic rescue. Lead author Tate Tunstall, of the San Diego Zoo Institute for Conservation Research, emphasized the importance of this finding, stating "the SWR went through a severe genetic bottleneck, but is now the most populous of all forms of rhino at ~20,000 individuals, suggesting that a genetic rescue utilizing these cell lines could be the foundation for a similar recovery in the NWR."
This work presents the first genome sequence of the NWR and thus the current, albeit limited, gene pool of this species. Tunstall and colleagues propose that this knowledge can help guide a tailored recovery program for the NWR. "Our study demonstrates the emerging role for whole-genome-sequencing analysis to evaluate the potential for population recovery," said Cynthia C. Steiner, who directed the study. Furthermore, advanced sequencing technologies, cryopreservation efforts like that of the San Diego Zoo Frozen Zoo, as well as novel reproductive strategies can be developed to improve recovery efforts for the NWR and other species that face similar threats of extinction. Recent efforts to this end are promising. The first pregnant SWR from artificial insemination has been reported by the San Diego Zoo Institute for Conservation Research, and it is hoped that surrogate SWR mothers may someday give birth to NWR progeny.
Researchers from the San Diego Zoo Institute for Conservation Research, Veterinary College, University of London, Dvůr Králové Zoo, and the University of California, Santa Cruz contributed to this work. The study was funded by grants from the Seaver Institute and the Caesar Kleberg Foundation for Wildlife Conservation.
The authors are available for more information by contacting Christina Simmons, San Diego Zoo public relations team; +1-619-318-3348).
Interested reporters may obtain copies of the manuscript via email from Dana Macciola, Administrative Assistant, Genome Research (email@example.com; +1-516-422-4012).
About the article:
The manuscript will be published online ahead of print on 24 May 2018. Its full citation is as follows: Tunstall T, Kock R, Vahala J, Diekhans M, Fiddes I, Armstrong J, Paten B, Ryder O, and Steiner C. 2018. Evaluating recovery potential of the Northern White Rhinoceros from cryobanked cells. Genome Research doi: 10.1101/gr.227603.118
About Genome Research:
Launched in 1995, Genome Research is an international, continuously published, peer-reviewed journal that focuses on research that provides novel insights into the genome biology of all organisms, including advances in genomic medicine. Among the topics considered by the journal are genome structure and function, comparative genomics, molecular evolution, genome-scale quantitative and population genetics, proteomics, epigenomics, and systems biology. The journal also features exciting gene discoveries and reports of cutting-edge computational biology and high-throughput methodologies.
About Cold Spring Harbor Laboratory Press:
Cold Spring Harbor Laboratory Press is an internationally renowned publisher of books, journals, and electronic media, located on Long Island, New York. Since 1933, it has furthered the advance and spread of scientific knowledge in all areas of genetics and molecular biology, including cancer biology, plant science, bioinformatics, and neurobiology. The Press is a division of Cold Spring Harbor Laboratory, an innovator in life science research and the education of scientists, students, and the public. For more information, visit our website at http://cshlpress.
Genome Research issues press releases to highlight significant research studies that are published in the journal.