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Cyborg bacteria outperform plants when turning sunlight into useful compounds (video)

American Chemical Society

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IMAGE: Artist's rendering of bioreactor (left) loaded with bacteria decorated with cadmium sulfide, light-absorbing nanocrystals (middle) to convert light, water and carbon dioxide into useful chemicals (right). view more 

Credit: Kelsey K. Sakimoto

WASHINGTON, Aug. 22, 2017 -- Photosynthesis provides energy for the vast majority of life on Earth. But chlorophyll, the green pigment that plants use to harvest sunlight, is relatively inefficient. To enable humans to capture more of the sun's energy than natural photosynthesis can, scientists have taught bacteria to cover themselves in tiny, highly efficient solar panels to produce useful compounds.

The researchers are presenting their work today at the 254th National Meeting & Exposition of the American Chemical Society (ACS). ACS, the world's largest scientific society, is holding the meeting here through Thursday. It features nearly 9,400 presentations on a wide range of science topics.

A brand-new video on the research is available at http://bit.ly/acscyborg.

"Rather than rely on inefficient chlorophyll to harvest sunlight, I've taught bacteria how to grow and cover their bodies with tiny semiconductor nanocrystals," says Kelsey K. Sakimoto, Ph.D., who carried out the research in the lab of Peidong Yang, Ph.D. "These nanocrystals are much more efficient than chlorophyll and can be grown at a fraction of the cost of manufactured solar panels."

Humans increasingly are looking to find alternatives to fossil fuels as sources of energy and feedstocks for chemical production. Many scientists have worked to create artificial photosynthetic systems to generate renewable energy and simple organic chemicals using sunlight. Progress has been made, but the systems are not efficient enough for commercial production of fuels and feedstocks.

Research in Yang's lab at the University of California, Berkeley, where Sakimoto earned his Ph.D., focuses on harnessing inorganic semiconductors that can capture sunlight to organisms such as bacteria that can then use the energy to produce useful chemicals from carbon dioxide and water. "The thrust of research in my lab is to essentially 'supercharge' nonphotosynthetic bacteria by providing them energy in the form of electrons from inorganic semiconductors, like cadmium sulfide, that are efficient light absorbers," Yang says. "We are now looking for more benign light absorbers than cadmium sulfide to provide bacteria with energy from light."

Sakimoto worked with a naturally occurring, nonphotosynthetic bacterium, Moorella thermoacetica, which, as part of its normal respiration, produces acetic acid from carbon dioxide (CO2). Acetic acid is a versatile chemical that can be readily upgraded to a number of fuels, polymers, pharmaceuticals and commodity chemicals through complementary, genetically engineered bacteria.

When Sakimoto fed cadmium and the amino acid cysteine, which contains a sulfur atom, to the bacteria, they synthesized cadmium sulfide (CdS) nanoparticles, which function as solar panels on their surfaces. The hybrid organism, M. thermoacetica-CdS, produces acetic acid from CO2, water and light. "Once covered with these tiny solar panels, the bacteria can synthesize food, fuels and plastics, all using solar energy," Sakimoto says. "These bacteria outperform natural photosynthesis."

The bacteria operate at an efficiency of more than 80 percent, and the process is self-replicating and self-regenerating, making this a zero-waste technology. "Synthetic biology and the ability to expand the product scope of CO2 reduction will be crucial to poising this technology as a replacement, or one of many replacements, for the petrochemical industry," Sakimoto says.

So, do the inorganic-biological hybrids have commercial potential? "I sure hope so!" he says. "Many current systems in artificial photosynthesis require solid electrodes, which is a huge cost. Our algal biofuels are much more attractive, as the whole CO2-to-chemical apparatus is self-contained and only requires a big vat out in the sun." But he points out that the system still requires some tweaking to tune both the semiconductor and the bacteria. He also suggests that it is possible that the hybrid bacteria he created may have some naturally occurring analog. "A future direction, if this phenomenon exists in nature, would be to bioprospect for these organisms and put them to use," he says.

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He acknowledges funding from the U.S. Department of Energy and the National Science Foundation.

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Title

Cyborg bacteria: Inorganic-biological hybrid organisms for solar-to-chemical production

Abstract

Though few CO2 reduction approaches can rival the energy efficiency and selectivity of biological CO2 fixation, the limited light absorption of natural photosynthesis pales in comparison to that of inorganic semiconductor based photovoltaics. Augmenting the CO2 reduction of biological organisms with inorganic light harvesting combines the best of both worlds. Bacteria, such as acetogens, fix CO2 into acetic acid. This common organic substrate is readily upgraded to a number of fuels, polymers, pharmaceuticals, and commodity chemicals through complementary genetically engineered bacteria, a process poised to supplant traditional petrochemical routes to the same chemicals. While this biological approach mitigates the high energy and material costs of traditional abiotic catalysts, the manufacturing of an inorganic semiconductor light harvester still relies upon non-green fabrication techniques. To address this, this presentation details a completely biological approach to the synthesis and utilization of an inorganic semiconductor light harvester for CO2 reduction at high efficiencies. Upon addition of a simple Cd2+ salt and the sulfur amino acid cysteine, the non-photosynthetic Moorella thermoacetica self-photosensitizes through the synthesis of light absorbing CdS nanoparticles. This hybrid organism, M. thermoacetica-CdS, produces acetic acid from CO2, water, and light at quantum efficiencies above 80%. This biological nature of this system renders it both self-replicating and self-regenerating, extending its operating lifetime and recycling all waste products to make this a zero-waste technology. Spectroscopic insight into the mechanism of the inorganic-biological charge transfer drives the development of subsequent generations of this technology, improving stability and performance. These advances and variations of the inorganic-biological hybrid organism concept drive this work towards outcompeting traditional approaches to chemical production, and augment the ever expanding portfolio of next generation green technologies.

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