CU researchers develop model for studying rare polio-like illness

AURORA, Colo. (Feb. 23, 2017) - Scientists, led by researchers at the University of Colorado School of Medicine, have developed the first animal model for studying paralysis caused by virus linked to a polio-like illness that paralyzed 120 children in 2014.

Working with mice in the laboratory of Kenneth Tyler, MD, chairman of the Department of Neurology, Alison Hixon, an MD/PhD candidate at the CU School of Medicine, and a team of researchers were able to demonstrate that several strains of the virus, known as enterovirus D68, or EV-D68, recovered during the 2014 epidemic can cause a paralytic illness in mice that resembles several aspects of the human cases.

"This is a really important breakthrough as it gives us a model to both study therapeutics and to understand how the disease develops," Tyler said. The results are published in the Feb. 23 edition of PLOS Pathogens, a peer-reviewed online open-access journal.

In the fall and winter of 2014, the United States experienced an epidemic of acute flaccid myelitis (AFM) cases in children coincident with a nationwide outbreak of EV-D68 respiratory disease. EV-D68 had previously been a rare cause of illness in the United States. Up to half of the 2014 AFM patients had RNA from EV-D68 detected in their respiratory secretions. The connection between EV-D68 and AFM led to the current research studies.

AFM appears as sudden onset of limb weakness, similar to polio, and medical imaging tests often reveal damage within the nervous system, particularly in the spinal cord. The effects of AFM can be devastating, leaving victims permanently disabled. Since 2014, the Centers for Disease Control and Prevention has been actively investigating the illness and has continued to receive reports of sporadic cases of AFM. In 2016, a total of 132 people in 37 states across the country were confirmed to have AFM.

"There are currently no established treatments for EV-D68," said Hixon. "A mouse model is an important first step in screening potential drug and vaccine therapies. Our results suggest that that there may be potentially effective strategies to treat or prevent EV-D68 and that's particularly important because we saw a surge in AFM cases in 2016."

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In addition to Hixon and Tyler, the co-authors of the article are Guixia Yu of the University of California San Francisco, J. Smith Leser of the University of Colorado School of Medicine, Shigeo Yagi of the California Department of Public Health, Penny Clarke, PhD, of the University of Colorado School of Medicine and Charles Y. Chiu, MD, PhD, of the University of California San Francisco.

The research was supported by grants from the U.S. National Institutes of Health and U.S. Department of Veterans Affairs. It was also supported in part by grants from the University of California San Francisco (UCSF)-Abbott Pathogen Discovery Award. The funders had no role in study design, data collection and analysis, decision to publish, or preparation of the manuscript.

Faculty at the University of Colorado School of Medicine work to advance science and improve care. These faculty members include physicians, educators and scientists at University of Colorado Health, Children's Hospital Colorado, Denver Health, National Jewish Health, and the Denver Veterans Affairs Medical Center. The school is located on the Anschutz Medical Campus, one of four campuses in the University of Colorado system. To learn more about the medical school's care, education, research and community engagement, visit its web site.