image: After cochlear microinjection of recombinant lentivirus carrying extrinsic Atoh1 and enhanced green fluorescent protein (EGFP), expression of EGFP displayed green fluorescence and expression of myosin 7a displayed red fluorescence. Long arrows represent hair cells and short arrows represent supporting cells. view more
Credit: Neural Regeneration Research
A recent study published in the Neural Regeneration Research (Vol. 8, No. 17, 2013) injected recombinant lentivirus carrying enhanced green fluorescent protein and the target gene Atoh1 into the cochlea of normal rats using a postauricular approach via the round window membrane. After 30 days, recombinant lentivirus was shown to have no impact on the hair cell numbers and auditory functions, infect hair and supporting cells, and promote supporting cells to transdifferentiate into hair cells. Researchers believed that it may be a new approach for gene therapy in the treatment of sensorineural hearing loss caused by damage to hair cells in the inner ear.
Article: " Lentivirus carrying the Atoh1 gene infects normal rat cochlea," by Song Pan1, Jingzhi Wan2, Shaosheng Liu3, Song Zhang1, Hao Xiong4, Jun Zhou1, Wu Xiong1, Kunfei Yu1, Yong Fu5 (1 Department of Otolaryngology, Guangming New District People's Hospital, Shenzhen 518106, Guangdong Province, China; 2 Department of Surgery, Hubei University of Science and Technology, Xianning 437100, Hubei Province, China; 3 Department of Otolaryngology, Beilun Branch of the First Affiliated Hospital, College of Medicine, Zhejiang University, Ningbo 315800, Zhejiang Province, China; 4 Department of Otolaryngology, Second Affiliated Hospital, Sun Yat-sen University, Guangzhou 510120, Guangdong Province, China; 5 Department of Otolaryngology, First Affiliated Hospital, College of Medicine, Zhejiang University, Hangzhou 310003, Zhejiang Province, China)
Pan S, Wan JZ, Liu SS, Zhang S, Xiong H, Zhou J, Xiong W, Yu KF, Fu Y. Lentivirus carrying the Atoh1 gene infects normal rat cochlea. Neural Regen Res. 2013;8(17):1551-1559.
Contact: Meng Zhao
eic@nrren.org
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Neural Regeneration Research
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Journal
Neural Regeneration Research