News Release

How daughter is different from mother in yeast cells

Press release from PLoS Biology

Peer-Reviewed Publication

PLOS

The mother-daughter relationship can be difficult to understand. Why are the two so different? In a new study published in PLoS Biology, Eric Weiss and colleagues show how this happens. In yeast cells, that is.

A research team has discovered a new mechanism for cell fate determination -- how one cell, the daughter, becomes dramatically different from the mother, even though they have the same genetic material. The study shows why mothers and daughters differ in how they express their genes.

The knowledge could lead to a better understanding of healthy human cells, what goes awry in cancer cells and how human stem cells and germ cells work. The fundamental mechanisms for cell division control in yeast are very similar to those in mammals.

When a yeast cell divides it produces a mother cell and a smaller, different daughter cell. The daughter cell is the one that actually performs the final act of separation, cutting its connection to the mother cell. And the daughter takes longer than the mother to begin the next cycle of division, since it needs time to grow up.

The key to the researchers' discovery of how this differentiation works is the gene regulator Ace2, a protein that directly turns genes on. The researchers found that the protein gets trapped in the nucleus of the daughter cell, turning on genes that make daughter different from mother.

The team is the first to show that the regulator is trapped because a signaling pathway (a protein kinase called Cbk1) turns on and blocks Ace2 from interacting with the cell's nuclear export machinery. Without this specific block, the machinery would move the regulator out of the nucleus, and the daughter cell would be more motherlike -- not as different.

"Daughter-cell gene expression is special, and now we know why," said Eric L. Weiss, assistant professor of biochemistry, molecular biology and cell biology in Northwestern's Weinberg College of Arts and Sciences. Weiss led the research team, which included scientists from the Massachusetts Institute of Technology.

The researchers also found that the differentiation of the mother cell and daughter cell -- this trapping of the regulator in the daughter nucleus -- occurs while the two cells are still connected.

By studying yeast, whose entire genome is known, scientists can learn the basics of cell division and apply that knowledge to the human system. Many of the fundamental mechanisms for cell division in yeast are conserved, or very similar, in mammals; many of the proteins involved in human disease are related to proteins that are involved in yeast cell division.

"Cancer may reflect a partial and aberrant loss of differentiated character, in which cells that were formerly specified to perform a specific task 'forget' that, and become more like the rapidly dividing stem cells from which they came," said Weiss. "Understanding how differentiated states are specified might help us figure out how to remind cancer cells to go back to their original tasks or fates -- or, more likely, die."

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Citation:Mazanka E, Alexander J, Yeh BJ, Charoenpong P, Lowery DM, et al. (2008) The NDR/LATS family kinase Cbk1 directly controls transcriptional asymmetry. PLoS Biol 6(8): e203. doi:10.1371/journal.pbio.0060203

PLEASE ADD THE LINK TO THE PUBLISHED ARTICLE IN ONLINE VERSIONS OF YOUR REPORT: http://biology.plosjournals.org/perlserv/?request=get-document&doi=10.1371/journal.pbio.0060203

PRESS ONLY PREVIEW OF THE ARTICLE: http://www.plos.org/press/plbi-06-08-weiss.pdf

CONTACT:
Eric L. Weiss
Northwestern University
Department of Biochemistry, Molecular Biology, and Cell Biology
Evanston, IL 60208-3500
+1-847-491-7034
elweiss@northwestern.edu


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