The protein Shp2 is required for normal growth factor and cytokine signaling. Mutations in Shp2 cause Noonan Syndrome (NS), which is associated with an increased risk of juvenile myelomonocytic leukemia (JMML), and Shp2 mutations have been associated with sporadic JMML as well as other leukemias. Dr. M. Golam Mohi, a research fellow from the Neel Laboratory at Beth Israel Deaconess Medical Center, in collaboration with research groups at Brigham and Women's Hospital and Emory University, examined the effect of Shp2 mutants on bone marrow and blood cell proliferation. Leukemia-associated Shp2 mutations caused a fatal leukemia in mice that resembled JMML. The leukemia-associated Shp2 mutants were much more likely to transform bone marrow cells than NS mutants were. The researchers also discovered that Shp2 mutants exhibited a perturbation in Ras signaling, a well-established pathway that plays a key role in governing cell survival and proliferation. Specific inhibitors of Ras/Mek and Tor pathways interfered with mutant Shp2-induced proliferation of abnormal bone marrow progenitor cells, which generate blood cells.
The results presented here establish a causal link between Shp2 mutations and leukemia, and point to leukemia-associated Shp2 mutants as attractive therapeutic targets for human cancers associated with Shp2 mutation. "Current treatments for leukemia caused by Shp2 mutations are often ineffective. Given the frequently lethal outcome of Shp2-associated cancers, clinical trials of currently available Ras/Mek and/or Tor inhibitors should be considered; at least until selective Shp2 inhibitors are developed. Our mouse model of Shp2-evoked myeloproliferative disorder may provide a useful system to test these and other therapeutic approaches," offers Dr. Mohi.
M. Golam Mohi, Ifor R. Williams, Charles R. Dearolf, Gordon Chan, Jeffery L. Kutok, Sarah Cohen, Kelly Morgan, Christina Boulton, Hirokazu Shigematsu, Heike Keilhack, Koichi Akashi, D. Gary Gilliland and Benjamin G. Neel: "Prognostic, therapeutic, and mechanistic implications of a mouse model of leukemia evoked by Shp2 (PTPN11) mutations"
The researchers include M. Golam Mohi, Gordon Chan, Heike Keilhack, and Benjamin G. Neel at Beth Israel Deaconess Medical Center in Boston; Ifor R. Williams at Emory University in Atlanta; Charles R. Dearolf at Massachusetts General Hospital in Boston; Jeffery L. Kutok, Sarah Cohen, Kelly Morgan, Christina Boulton, and D. Gary Gilliland at Brigham and Women's Hospital in Boston; and Hirokazu Shigematsu and Koichi Akashi at Dana-Farber Cancer Institute in Boston. This work was supported by NIH grants and grants from the Leukemia and Lymphoma Society. D.G.G. is an investigator of the HHMI. M.G.M was supported by a Hood Postdoctoral Fellowship from the Medical Foundation.
Publishing in Cancer Cell, Volume 7, Number 2, February 2005, pages 179-191. http://www.cancercell.org/
Journal
Cancer Cell