News Release

Proteins that bind to sperm offer clues to male fertility and possible male contraception

Peer-Reviewed Publication

University of North Carolina Health Care

CHAPEL HILL -- The discovery by University of North Carolina scientists of a distinct family of proteins that bind to human sperm may offer important new clues to male fertility and possible male contraception.

A report of the new findings appears in the March issue of the journal Endocrinology.

The three proteins in the newly identified HE2 family are secreted in the epididymis, a coiled tubular network several yards long attached to the top of each testicle. As they mature, sperm pass through the epididymis and into another tube, the vas deferens, before emptying through the urethra during ejaculation.

Compared to the testes, the epididymis has been overlooked on a molecular level until very recently. Just five years ago, in 1995, it was considered by some as not essential for the development of sperm's fertilizing ability.

"Now it's known that sperm that have not passed through the epididymis fertilize an egg very poorly if at all. And after they pass through, they're more ready to fertilize an egg," said Susan Hall, PhD, research assistant professor of pediatrics at UNC-Chapel Hill School of Medicine. "In the epididymis, sperm go through certain maturational steps that are not very well understood at this point. That's why we're studying proteins that are synthesized in the epididymis and secreted into that tubule that might possibly interact with sperm in such a way to affect their maturation."

Attempting to fill in the gaps in epididymis molecular biology and to find possible protein targets for male contraception, Hall and her colleagues at UNC's Laboratories of Reproductive Biology began searching for genes expressed specifically in that organ.

Armed with grants from the privately endowed Contraceptive Research and Development Program (CONRAD), the Andrew Mellon Foundation, the NIH, and the Fogarty International Center, the UNC researchers found that three categories of HE2 epididymal proteins -- alpha, beta, and gamma -- were all regulated by the male hormone testosterone.

These androgen-regulated, epididymis-specific proteins are unique, having no known homologues. A DNA database search turned up nothing similar. This would imply their function is unique, "which is why we feel it's another hint that they're involved in fertility," Hall said.

The proteins also appear to be "conserved" in that the family has been identified in monkeys.

"We know two occur on the surface of sperm, so they're in a position to function in sperm maturation, although we don't know for certain that's the case" Hall said. She and her co-authors also noted that the demonstration of HE2beta on ejaculated sperm "further supports a direct role in fertilization."

Further work aimed at discovering exactly what these epididymis-specific proteins do may lead to identifying one or more potential targets for a male contraceptive. More specifically, they might offer the source of a potential antigen (protein target) for a contraceptive vaccine. More effective methods of contraception are urgently needed to prevent the enormous overgrowth of the world's population that is expected to occur over the next half century.

###

Note to media: Contact Dr. Hall at 919-966-0924; email: shh@med.unc.edu

UNC School of Medicine contact: Karen Stinneford, 919-966-6047; email: kstinnef@unch.unc.edu Contact Leslie H. Lang at 919-843-9687; email: llang@med.unc.edu


Disclaimer: AAAS and EurekAlert! are not responsible for the accuracy of news releases posted to EurekAlert! by contributing institutions or for the use of any information through the EurekAlert system.