Pet dogs suffering bladder cancer, for which all other approaches failed, were treated with a Purdue University experimental approach. A: Bladder cystoscopic biopsy being performed in patient dog to investigate tumor characteristics. B: Ultrasound image obtained to record tumor mass dimensions. Sagittal view of the bladder. Arrows point to mass in the neck of the bladder. C: Presence of Epidermal Growth Factor (EGF) Receptor on bladder cancer cells was revealed by binding and uptake of fluorescently labeled EGF (red). Nucleus (blue) and actin cytoskeleton (green) are also shown.