Housed inside a commercial cell culture incubator (A) are separate Plexiglas pressure chambers (B). Pressure is generated via compressed breathing air with 5% CO2that is controlled by a dual stage regulator (C) connected to manifold (D) which distributes the compressed air to separate pressure valves (E) that deliver compressed air to the pressure chambers. Flow rates are further controlled by pinch valves (G) connected to outflow lines. Pressure inside each of the chambers is monitored and recorded using data logging pressure gauges (F) that are connected to a computer (H) for data collection.
Reprinted from Journal of Neuroscience Methods, Smith, Michael and Eskandari, Ramin. A novel technology to model pressure-induced cellular injuries in the brain. 293 (2018) 247-253. Epub October 6, 2017, with permission from Elsevier.