This is the fluorescence of the dsx1 reporter strain. Nine-day-old fully matured daphniids were observed under bright-field (top row), GFP filter (middle row), and mCherry filter (bottom row). Ubiquitous green fluorescence is caused by the EF1α-1::h2b-gfp background genotype. In all cases, the red signal from the guts (dotted lines) represents the autofluorescence of chlorella, the main food used in daphniid cultivation. An1: first antennae. T1: first thoracic legs. Scale bars = 0.5 mm. Pictures were taken under the same camera settings.