Figure 1 A. Overall workflow for fast AST B. Individual cells are sucked into the cell channels where they get stuck at the 300 nm constriction at the end(inset) C1. One row of 2000 cell channels are treated with an antibiotic and the other row is used as a reference. C2. Growth in one individual cell channel without antibiotic (left) and one with antibiotic (right ) monitored over time (x-axis) as observed with phase contrast microscopy. C3. Length extension over time as determined for cells in 1600 individual cell channels without antibiotic (left) and with antibiotic (right). C4 Average growth rates for the bacteria in C3 together with 99.9% SEM and population standard deviation D. The average growth rate and 99.9% SEM for susceptible bacteria exposed to one of of nine different antibiotics (colors), normalized to the growth rate in the non-treated reference channels. Only data from one typical references channel is displayed(gray). dots indicate when the growth rate has dropped below untreated reference with 99.9% probability. "