Development of algorithms for automated analysis of neuronal survival assay using BioStation CT and CL-Quant. (IMAGE)
Caption
Development of algorithms for automated analysis of neuronal survival assay using BioStation CT and CL-Quant. (A) Workflow diagram showing the development of automated algorithms for neuronal survival analysis. iPSC-derived human neurons were transfected at DIV8 and maintained in BioStation CT with images taken every 12 h. The collected images were quantified by manual counting to create a reference data set for the subsequent development of automated algorithms. (B) Representative fluorescence and brightfield images acquired by the automated imaging system of BioStation CT. Neurons were transfected with red fluorescence tracer plasmid (pSF-MAP2-mApple). (C) Example of automated detection shows cell bodies in red, neurites in yellow and nodes in blue. The information can be plotted to show cell survival, neurite outgrowth and neuronal health over time in culture.
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Jeonghoon Choi, et al.
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