UVC microscopy enables label-free high-contrast imaging of nanoscale biological structures. (IMAGE)
Caption
a, absorption of light in biological materials is significantly increased in the deep UV, yet conventional optical materials also stop being transparent. b, a purpose-built UV microscope enables the recording of differential phase contrast images in the c-band of the UV regime, which can be processed into extinction coefficient maps. Shown here is the optical layout of the components of the microscope. c, a comparison between regular brightfield microscopy and the newly developed UVC microscope highlights the potential of the new technique in imaging nanoscale biological structures. Shown are liver sinusoidal endothelial cells; the tiny black spots within the membrane are fenestrations. Note that the nominal resolution of both techniques is the same.
Credit
by Florian Ströhl, Deanna L. Wolfson, Ida S. Opstad, Daniel H. Hansen, Hong Mao & Balpreet S. Ahluwalia
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Credit must be given to the creator.
License
CC BY