fluorescent visualization of proteins in leaf cells (IMAGE)
Caption
In addition to performing genetic and biochemical studies, the scientists used a green fluorescent protein (GFP) "tag" and microscope facilities at Brookhaven Lab's Center for Functional Nanomaterials to visualize the proteins they were studying in leaf cells. Left: Localization of a GFP-labeled electron donor protein along the endoplasmic reticulum (inner network of membranes) in leaf cells. Right: A GFP-labeled complex of the P450 enzyme interacting with the electron donor. In this case the scientists attached half of the GFP tag to each of these proteins; the fluorescent glow occurs only when the two halves come together as the proteins interact. (In both images the red signal comes from chlorophyl.)
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Brookhaven National Laboratory
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