Identification of mutations in M3C1 responsible for improved conjugation to S. cerevisiae. (IMAGE)

BioDesign Research

Identification of mutations in M3C1 responsible for improved conjugation to S. cerevisiae.

Caption

S. cerevisiae transconjugant colony formation on minimal plates lacking histidine following conjugation of control: pTA-Mob 2.0, M3C1; hybrid: M3C1/M3C2; or mutated pTA-Mob 2.0 Tp/To plasmids from E. coli. Plasmid schematics for M3C1_F1–F5 generated by swapping PCR-amplified fragments from M3C1 into M3C2 are shown. Green arrows represent fragments originating from M3C1 and gray arrows represent fragments originating from M3C2. Mutations in pTA-Mob 2.0 Tp/To plasmids are indicated by green stars in the schematic of traJ (Tp, a cluster of mutations; and To, a single mutation). Transconjugant colony counts are reported below the plate images.

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