OsAGO1d functions in phasiRNA biogenesis in rice anther (IMAGE)
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Spatial distribution and biogenesis of two types phasiRNAs. miR2118 specifically accumulates in anther wall cells, where 21-nt phasiRNA are produced. By contrast, 24-nt phasiRNA biogenesis occurs mainly in meiocytes, where miR2275 and OsAGO1d accumulate. During phasiRNA biogenesis, OsAGO1d binds miR2118 and miR2275 to form RNA-induced silencing complexes that mediate the initial cleavage of long non-coding precursor RNAs. The cleavageproducts are converted into double-stranded RNAs by RDR6 and further diced into 21- and 24-nt phasiRNAs by DCL4 and DCL3b/DCL5, respectively. 21-nt phasiRNAs with 5′U and 5′C can be loaded into OsAGO1d and MEL1 for execution. 24-nt phasiRNAs could be loaded into currently unknown AGO proteins and may mediate DNA methylation of target sites. Upon cold treatment, OsAGO1d is induced and required to sustain male fertility, as WT plants produce viable pollen but osago1d mutants produce inviable pollen.
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