Graphical explanation of ET-seq and DART (IMAGE)
Caption
To successfully edit genes within multiple members of a microbial community, UC Berkeley scientists had to develop two new methods: Environmental Transformation Sequencing (ET-Seq), top, which allowed them to assess the editability of specific microbes; and DNA-editing all-in-one RNA-guided CRISPR-Cas transposase (DART), which allowed highly specific targeted DNA insertion into a location in the genome defined by a guide RNA. The DART system is barcoded and compatible with ET-Seq so that, when used together, scientists can insert, track and assess insertion efficiency and specificity.
Credit
Jill Banfield lab, UC Berkeley
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