homo-FRET new tool for observing bacterial signal processing in real time (IMAGE)
Caption
Illustration of Rice University’s “homo-FRET” method for observing real-time phosphorylation reactions in two-component sensory systems in live bacteria. Specific stimuli outside the cell (top) initiate phosphorylation (middle), which activate response regulator proteins that form pairs (bottom right) to produce a biochemical cascade that ultimately changes the cell’s behavior. To observe phosphorylation in real-time, Rice researchers engineered strains of E. coli to produce green fluorescent tags that depolarize light from an excitation laser only when they interact as pairs (bottom right). (Figure courtesy of Ryan Butcher/Rice University)
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Ryan Butcher/Rice University
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