Demonstration of a correlation between arrangements of actin filaments and nuclear position. (IMAGE)
Caption
Actin filaments and nuclei information was isolated using non-overlapping fluorophores (Alexa Fluor 488 and SyTOX Deep Red). Then, 80% of the dataset of filaments was used as input to train a transformer-based network using the corresponding paired nuclei to evaluate the proximity to the real solution. The process iteration resulted in a fully trained network that was then used to generate the nuclei of the remaining 20% filament images of the dataset. The generated nuclei and their real counterparts were identified, and the coordinates of their centroids were determined to evaluate the network’s ability to predict the nuclear position using only actin filament arrangements.
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SUTD
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