Scheme figure of MOPCS in this study (IMAGE)
Caption
Within the whole genome sequence of SARS-CoV-2, a highly conserved region of N sequence was selected to detect the positive samples, and a region of S sequence with three featured mutation sites of Delta, Omicron, and BA.1 variants was selected to distinguish the variants of positive samples. After the RNA sequences were extracted, purified, and reverse-transcribed into double-strand DNA templates, the templates were mixed with Cas12a-crRNA complexes. crRNAs were designed to target the conserved region, D950N, N969K, and L981F mutation sites. After loading onto the SPR chips with pre-immobilized ssDNA reporter, only if the DNA templates contain the exact same sequences with the crRNA, could the Cas12a be activated and the ssDNA reporter was trans-cleaved, inducing a changed (decreased) SPR wavelength.
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