Integrated single-cell transcriptome landscape and CNV of bone marrow cells in multiple myeloma and normal controls. (IMAGE)
Caption
A: The t-distributed stochastic neighbour embedding (t-SNE) plot demonstrates the cell types in bone marrow (BM). B: The box plot shows the different percentages of each type of BM cell in normal controls and multiple myeloma (MM) patients. C: t-SNE representation of plasma cells in BMbetween normal and MM patients. D: Heat map showing large-scale copy number variations (CNVs) of plasma cells from MM patients and normal controls. The normalised CNV levels are shown. The red colour represents a high CNV level, and blue represents a low CNV level, where each column is a chromosome and each row is a cell. Row side-colour annotated cells originating from each person and classified as normal or tumour. E: Violin plots showing distributions of CNV scores among different stages of plasma cells (***P<0.0001). F: Interphase FISH of cells from stage III patients. Samples had at least 80% tumour purity based on SDC1+. Representative cells are shown. G: Expression patterns of representative markers discriminating normal and malignant plasma cells plotted onto the t-SNE map. The colour key from grey to red indicates relative expression levels from low to high. H: KEGG analysis shows the top upregulated pathways in stage III disease tissues compared with normal tissues.
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