DNA nicks induce efficient HTR (IMAGE)
Caption
Restorative gene editing using sequences from the counterpart chromosome: The standard CRISPR enzyme Cas9 offers the ability to make repairs but also potentially results in unintended mutations (mutagenic events) at the targeted site and possibly elsewhere in the genome (left). In contrast, the nickase enzyme results in more efficient gene correction and no mutagenic events (right).
Credit
Guichard/Bier
Usage Restrictions
Must be used in conjunction with new soft CRISPR technology paper.
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Original content