The workflow of high-throughput intact glycopeptides quantitation strategy (HTiGQs) (IMAGE)
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The workflow of high-throughput intact glycopeptides quantitation strategy includes protein extract, preparation of TMT and DMEN-labeled glycopeptides, stepped collision energies HCD followed bynproduct-dependent ETD (sceHCD-pd-ETD) acquisition for intact N-glycopeptide identification and quantification and the bioinformation analysis for biomarker discovery, healthy controls (A), HBV patients (B), CIR patients (C), and HCC patients (D).
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