Figure 1: Morphological Features of Ontogenic and Regenerating Scales (IMAGE)

Kanazawa University

Figure 1: Morphological Features of Ontogenic and Regenerating Scales

Caption

(a) A goldfish and a binocular view of an ontogenic scale. Grooves radiate from the center focus. (b) Binocular views of a regenerating scale on day 14 (left) and the same scale stained for TRAP activity, indicated by the red color along the grooves (right). The grooves formed a mesh-like structure at the center of the goldfish scales. (c) Light microscopic views of the surface on the osseous layer of the regenerating goldfish scales cultured for 86 h at 22°C on the ground. TRAP staining (left) and F-actin staining (right) superimposed with DAPI staining of the nuclei. A TRAP-positive multinucleated osteoclast possessed an actin ring along the groove. (d) Schematic illustration of morphological features of goldfish scales. Fish scales can regenerate following scale removal. On the groove edge of the goldfish scales, a multinucleated osteoclast exhibited a well-developed RB and CZs (e). Active cuboidal osteoblasts (f) are observed at the periphery and on the dermis side of the scale. They represent a highly-developed rER and Go. OB, osteoblast; OC, osteoclast; TRAP, tartrate-resistant acid phosphatase; DAPI, 4', 6-diamidino-2-phenylindole; N, nucleus; RB, ruffled border; CZ, clear zone; rER, rough endoplasmic reticulum; Go, Golgi apparatus. Scale bars = 1 mm in a and b, 10 μm in c, 5 μm in e and f.

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Kanazawa University

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