Immune response of Kupffer cells depending on the molecular mobility of polyrotaxane (PRX) surfaces. (IMAGE)

Tokyo Medical and Dental University

Immune response of Kupffer cells depending on the molecular mobility of polyrotaxane (PRX) surfaces.

Caption

LPS-stimulated mouse Kupffer cells on PRX surfaces with high mobility had smaller areas of spread than those on PRX surfaces with low mobility, whereas the cells showed higher expression levels of Toll-like receptor 4 (Tlr4), M1 marker and pro-inflammatory cytokine genes, and frequently formed multiple cytoplasmic vacuoles in the their cytoplasms. In contrast, the LPS-stimulated Kupffer cells on PRX surfaces with low mobility had wider areas of spread, enhancing the expression of M2 marker and the Yap genes, while decreasing the expression of the pro-inflammatory genes. These results suggest that the highly mobile surfaces favor the M1 polarization of Kupffer cells, whereas less mobile surfaces favor the M2 polarization.

Credit

Department of Organic Biomaterials,TMDU

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