Graphical Abstract (IMAGE) Stowers Institute for Medical Research Caption The development of mRNA knock-down technologies for use in vertebrate organisms such as zebrafish has been limited. CRISPR-RfxCas13d has been identified as an effective method for the systematic study of gene function in vivo during embryogenesis across a range of animal species. Credit Bazzini Lab, Stowers Institute Usage Restrictions None License Licensed content Disclaimer: AAAS and EurekAlert! are not responsible for the accuracy of news releases posted to EurekAlert! by contributing institutions or for the use of any information through the EurekAlert system.