Electron Micrographs of Replisome (IMAGE)

DOE/Brookhaven National Laboratory

Electron Micrographs of Replisome

Caption

A series of electron micrographs show the barrel-shaped helicase with several components of the replisome: a) helicase (grey) with Ctf4 (light blue), which is a recruitment factor for the DNA-replication primase-polymerase, b) helicase, Ctf4, and primase-polymerase (dark blue), which works with the back-end polymerase, c) helicase, Ctf4, and front-end polymerase (green), d) helicase and front-end polymerase, e) helicase, Ctf4, front-end polymerase, and back-end primase-polymerase (dark blue). Panels a-c used an unprimed short DNA fork, and d-e used a longer and primed DNA fork. The electron micrographs show that the replisome is a 20-nanometer sized nanomachine.

Credit

Brookhaven National Laboratory

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