Resistance to Sedation (IMAGE)
Caption
A. The Dunc13P84200/+ heterozygotes require a greater time to reach 50% loss-of649 righting (T1/2 LOR) reflex levels. (***p<0.001, n = 17). B. The concentration of ethanol was determined in Dunc13P84200/+ and control flies exposed to 50% ethanol vapor for 0, 15, 30, or 45 min; no significant differences were found (p>0.05, n=6). C. The ability of Dunc13P84200/+ and control flies to metabolize ethanol was determined by first exposing flies to ethanol vapor for 45 min, and then by measuring the ethanol remaining in the flies 0, 30, 60, 120 min after the exposure. No significant differences in ethanol metabolism were detected at each time point (t=0.037, p>0.05, n=6). D. The neural expression of the Dunc13KK101383 RNAi transgene led to significantly slower T1/2 LOR compared to the genotype controls (***p<0.05, n = 10). E. The induced neural expression of the Dunc13JF02440 RNAi transgenes also led to a significantly slower T1/2 LOR as compared to the within genotype control (p <0.01, n =8). Induction was accomplished with a 24 hr, 30 °C heat treatment, followed by a 3 hr recovery period at room temperature. F. Inducing the expression of a wild type Munc13-1 cDNA for 48 hrs led to a significant decrease in LOR for the Dunc13P84200/+ flies (***p=0.001, N=9). Induction was accomplished with a 48 hr, 30 °C heat treatment, followed by a 3 hr recovery period at room temperature. G. Munc13-1::EGFP is co-localized with Bruchpilot, a protein localized to presynaptic active zones, in the presynaptic compartment of the larval neural muscular junction.
Credit
Xu et al., <i>eNeuro</i> (2018)
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