Imnunophenotype (IMAGE)
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(A) Immunophenotype of native newly isolated hADSCs (passage 1). Cells were positive for CD29, CD44, CD73, CD90, CD105, and negative for (Negative control) CD11b, CD19, CD34, CD45, and HLA-DR. (B,C) Osteogenic, adipogenic and chondrogenic differentiation of hADSCs. Phase contrast microscope images. To differentiate toward adipogenic lineage native hADSCs were cultured in reprogramming medium for 14 days. At day 14, cells were fixed and stained with Oil Red O. For osteogenic differentiation cells were cultured in reprogramming medium for 28 days. At day 28, cells were fixed and analyzed by von Kossa staining. Chondrogenic differentiation was determined by staining with Alcian blue on day 21 after seeding (osteogenic differentiation: scale bar, 100 μm; adipogenic and chondrogenic differentiation: scale bar, 200 μm). The quantitative analysis of relative mRNA expression of RUNX2, PPAR-γ and Col2α1 genes, associated with osteogenic, adipogenic and chondrogenic differentiation, respectively, was also carried out. Both the specific staining and gene expression analysis confirmed the differentiation capacity of hADSCs.
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Kazan Federal University
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