Figure. Reverse genetics for SARS-CoV-2 by the CPER method (IMAGE)

Osaka University

Figure. Reverse genetics for SARS-CoV-2 by the CPER method

Caption

A total of nine gene fragments covering the full-length SARS-Cov-2 genome and a linker fragment were amplified. Because all the fragments were designed to include ends that overlapped with the adjacent fragments, they could be assembled as a circular viral genome by an additional PCR step. Recombinant SARS-CoV-2 was rescued by transfection of the circular viral genome into susceptible cells. Cytopathic effects were only observed in cells transfected with CPER products.

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Osaka University

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