Fig.1. Cas9 Introduces a DNA Double-Strand Break in the Target Gene (IMAGE)

Osaka University

Fig.1. Cas9 Introduces a DNA Double-Strand Break in the Target Gene

Caption

Cas9 introduces a DNA double-strand break in the target gene, allowing the gene to be edited when a repair template is present. A drawback to the CRISPR/Cas9 system is that the cellular DNA repair machinery can introduce inaccuracies following a double-strand break (left panel). Nakajima et al report a new gene-editing method that avoids the formation of double-strand breaks. Cas9 nickase produces a combination of single nicks in the genome and the DNA carrying the repair template, resulting in precise and efficient gene editing (right panel).

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Osaka University

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