Protein architecture of transcription enhancer (IMAGE)
Caption
Researchers have precisely mapped the binding locations of over 400 proteins on the yeast genome using ChIP-exo. The method (top) uses an antibody to 'fish out' a specific DNA-bound protein such as a transcription factor (TF), then an exonuclease is used to define protein-DNA boundaries, and finally the location of the binding event in the genome is determined by sequencing the DNA to which the protein is bound. Mirrored peaks (bottom) from multiple ChIP-exo experiments show enrichment of DNA sequencing reads for a transcription factor and several cofactor proteins that interact with the TF to activate nearby genes along the two strands of the DNA helix (white), revealing the protein architecture at a yeast transcriptional enhancer.
Credit
Pugh Lab, Cornell and Mahony Lab, Penn State
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