Autophagy (IMAGE)
Caption
To determine whether specific genes play a role in autophagy in proliferating and quiescent cells, Hilary Coller's team reduces the activity of these genes and then determines the amount of autophagy within the cell. To assess autophagy levels in one previous experiment, Sarah Pfau, who earned her bachelor's degree from Princeton in 2008, exposed the cells to a fluorescently labeled antibody that recognizes a protein present on autophagosomes, the structures that cells form to perform autophagy. Areas within the cell where autophagy was taking place glowed green when observed under a fluorescent microscope.
Credit
Sarah Pfau
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