Dependence of proteolytic activity (IMAGE)

Kazan Federal University

Dependence of proteolytic activity

Caption

Dependence of proteolytic activity of subtilisin-like protease on the growth dynamics of P. pastoris recombinant yeast. a pPINK-HC-lysoz-aprBp and pPINK-LC-lysoz-aprBp; (b) pPINK-HC-killer-aprBp and pPINK-LC-killer-aprBp; (c) pPINK-HC-α-mat.factor-aprBp and pPINK-LC-α-mat.factor-aprBp; (d) determination of the specific activity of subtilisin-like protease by the hydrolysis of Z-Ala-Ala-Leu-pNa. 1, pPINK-HC-lysoz-aprBp; 2, pPINK-LC-lysoz-aprBp; 3, pPINK-HC-killer-aprBp; 4, pPINK-LC-killer-aprBp; 5, pPINK-HC-α-mat.factor-aprBp; 6, pPINK-LC-α-mat.factor-aprBp; K, culture fluid of recombinant yeast, before the addition of methanol

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Kazan Federal University

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