IBS Biologists Designed CRISPR-Cpf1 for Gene Editing in Soybean and Tobacco (IMAGE)
Caption
CRISPR-Cpf1 is introduced into plant cells without cell walls (protoplasts) as a pre-assembled complex of the protein Cpf1 (red scissors) and the CRISPR-RNA (crRNA, in blue). Cpf1 functions as gene scissors that cuts the plant's DNA (shown in grey) at specific locations indicated by the crRNA. In the case of soybean, the cut in two FAD2 genes increases the percentage of oleic acid in soybean seeds, resulting in a healthier oil. In wild tobacco, IBS scientists mutated a hormone biosynthetic gene to examine the function of this hormone in the plant development.
Credit
IBS
Usage Restrictions
None
License
Licensed content