Folate Biosynthesis (IMAGE) Johns Hopkins Medicine Caption The accompanying image illustrates the stages of enzyme activity of the first step of folate biosynthesis: free enzyme (orange), enzyme with substrate bound (salmon), and enzyme with pyrophosphate bound (gold), superimposed on a drawing of E. coli and the folate biosynthetic pathway. The free floating substrate is shown in blue, with the phosphates in red. Credit The rendition was contributed by Devon Nikasa an alumna of the Art as Applied to Medicine Program at Hopkins. Usage Restrictions Image may only be used with permission from Cell Press. License Licensed content Disclaimer: AAAS and EurekAlert! are not responsible for the accuracy of news releases posted to EurekAlert! by contributing institutions or for the use of any information through the EurekAlert system.