Hari Shroff and Patrick La Riviere with Dual-View Light Sheet Microscope (diSPIM) (IMAGE)
Caption
Marine Biological Laboratory (MBL) Fellows Patrick La Riviere of University of Chicago, left, and Hari Shroff of the National Institute of Biomedical Imaging and Bioengineering with the diSPIM system (Dual-view Selective Plane Illumination Microscopy) co-developed by Shroff's laboratory and Applied Scientific Instrumentation. Note the two objectives mounted at right angles above the sample. A light sheet is created from one objective and imaged through the other objective. A stack of images is created by moving the light sheet through the sample. Especially useful for live-cell imaging and developmental biology, the system provides rapid 3-D imaging with low phototoxicity to samples.
Credit
Diana Kenney
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