TR-LSCI for animal and human applications (IMAGE)
Caption
a, the diagram of transmissive-detected laser speckle contrast imaging (TR-LSCI) system. b, the diagram of conventional reflective-detected LSCI system. c, comparison between TR-LSCI and conventional LSCI for cutaneous/subcutaneous blood flow mapping on different parts of mice body. In (c), it is obvious that TR-LSCI has better imaging quality for each body parts, especially for thick parts. d, TR-LSCI for dynamic blood flow monitoring in the mice hindlimb after acetyl choline (a drug that dilates blood vessels) injection. TR-LSCI was capable of distinguishing individual cutaneous blood vessels. The plots in (d) represent the dynamics in the femoral vein and in the branch of femoral vein, which shows that, compared to femoral vein, the blood flow velocity in the branch of femoral vein responded stronger but recovered more quickly. e, comparison between TR-LSCI and conventional LSCI for subcutaneous blood flow mapping on different parts of human hands. As shown in (e), the blood flow in the individual blood vessels was resolvable in TR-LSCI, while conventional LSCI was not able to distinguish. f, conventional LSCI and TR-LSCI for reactive hyperemia experiments on human hands. Conventional LSCI was used to monitor superficial perfusion, while TR-LSCI was used to monitor blood flow in the deep vessels. The histograms in (f) represent the dynamics in the superficial perfusion and deep blood flow. The results suggested that blood flow in the deep larger vessels was more affected by pressure and recovered more slowly after pressure was released.
Credit
by Dong-Yu Li, Qing Xia, Ting-Ting Yu, Jing-Tan Zhu, and Dan Zhu
Usage Restrictions
Credit must be given to the creator.
License
CC BY